Molecular Physiology

Team

Dr. Judith Bossen

Postdoc

R. 812
Phone: +49 431 880-2578
Telefax: +49 431 880-4197
jbossen@zoologie.uni-kiel.de

Short CV

  • 2014 - 2017

PhD student, Kiel University, Germany

  • 2011 - 2014      
Master studies, Kiel University, Germany
  Thesis: "Physiologische Rolle der Acyloxyacylhydrolase in Dictyostelium discoideum"
  • 2008 - 2011
Bachelor studies, Kiel University, Germany
  Thesis: "Analyse der Lysozym-Synthese bei Dictyostelium discoideum in der Abhängigkeit von der Gegenwart von Bakterien"

 

   
   
   
   

 

Research topic

I am working on the development and establishment of a lung cancer model in Drosophila. The aim is to understand the molecular and genetic basis of tumor formation and progression. Overexpression of cancer-associated genes (e.g. EGFR, Ras, MAPK) in the tracheal epithelium can induce cancer-like phenotypes like epithelial meta- and hyperplasia. I am investigating this phenotypes by microscopic analysis for differences on a cellular level (quantification of epithelial thickness, cell and nucleus size and number, cell connections and cell shape…) and by other methods (IHC, qPCR) to investigate the tumor formation on a translational and transcriptional level.

Publications


Bossen J, Uliczka K, Steen L, Neugebauer P, Mai M.M-Q, Fink C, Stracke F, Heine H and Roeder T (2020)
Driver mutations in major lung cancer oncogenes can be analyzed in Drosophila models.
ALTEX, doi: 10.14573/altex.1912131

Bossen J, Uliczka K, Steen L, Pfefferkorn R, Mai MM-Q, Burkhardt L, Spohn M, Bruchhaus I, Fink C, Heine H, Roeder T (2019)
An EGFR-induced Drosophila lung tumor model identifies alternative combination treatments.
Mol Cancer Ther, accepted for publication

Uliczka K, Bossen J, Pfefferkorn R, Heine H and Roeder T (2015)
Etablierung eines Drosophila-Lungenkarzinom-Modells.
Pneumologie, 69-A7

Dr. Christine Fink

Postdoc

R.812
Phone: +49 431 880-2578
Telefax: +49 431 880-4197
cfink@zoologie.uni-kiel.de

Short CV

  • since 2012
Postdoc, Kiel University, Germany
  • 2008 - 2012
PhD student, Kiel University, Germany
  Thesis: "Die Architektur des intestinalen Immunsystems der Taufliege Drosophila melanogaster"
  • 7/2009 - 7/2010      
Maternal leave
  • 2002 - 2008
Diploma studies in Biology, Kiel University, Germany
  Thesis: "Der Einfluss des Hungerns auf die Transkriptionsaktivität isolierter Organe der Taufliege Drosophila melanogaster"
 
 

Research topics

Based on results of my phD thesis I am investiganting the epithelial immune response in the fruit flys intestine. Especially the interaction between the microbiome and the host is not fully understood yet. Beside the classical antimicrobial peptide response via NF-kB pathways (Toll & Imd), I am interested in the DUOX response mechanisms that not only effects invading pathogens but even also the own microbiome. I want to understand how the host-microbiome-homeostatis is staying in balance.

Furthermore I am working with the airway epithelium of Drosophila. I am investiganting different epithelial phenotypes in the fly that model lifestyle diseases like  Asthma, COPD & lung cancer and try to investigate the underlying cell signalling mechanism that may cause the typical disease-like phenotypes. One major aspect is to test the influence of human suceptibility genes in the fly model to further investigate targets for a better treatment of this diseases.

Publications

2020   Bossen J, Uliczka K, Steen L, Neugebauer P, Mai M.M-Q, Fink C, Stracke F, Heine H and Roeder T
Driver mutations in major lung cancer oncogenes can be analyzed in Drosophila models.
ALTEX, doi: 10.14573/altex.1912131

2020   Niu X, Fink C, Kallsen K, Mincheva V, Franzenburg S, Prange R, Bossen J, Heine H, Roeder T 
Balanced JAK/STAT signaling is critical to maintain the functional and structural integrity of the Drosophila respiratory epithelium.
BioRxiv doi: https://doi.org/10.1101/2020.06.19.160929

2020   Richert E, Papenkort J, Klettner A, Tode J, Koinzer S, Brinkmann R, Fink C, Roeder T, Lucius R, Roider J
Response of Retinal Pigment Epithelium (RPE)-Choroid Explants to Thermal Stimulation Therapy of the RPE (TSR)
Lasers in Surgery and Medicine, accepted for publication DOI:10.1002/lsm.23288

2020   Li Y, Romey-Glüsing R, Tahan Zadeh T, von Frieling J, Hoffmann J, Huebbe P, Bruchhaus I, Rimbach G, Fink C, Roeder T 
Furbellow (brown algae) extract increases lifespan in Drosophila by interfering with TOR-signalling.
Nutrients, 12:1172, doi:10.3390/nu12041172

2019 Bossen J, Uliczka K, Steen L, Pfefferkorn R, Mai MM-Q, Burkhardt L, Spohn M, Bruchhaus I, Fink C, Heine H, Roeder T 
An EGFR-induced Drosophila lung tumor model identifies alternative combination treatments.
Mol Cancer Ther, accepted for publication

2018 Prange R*, Thiedmann M*, Bhandari A, Mishra N, Sinha A, Häsler R, Rosenstiel P, Uliczka K, Wagner C, Yldirim AÖ, Fink C, Roeder T 
Drosophila model of cigarette smoke induced COPD identifies Nrf2 signaling as an expendient target for intervention
Aging, 10: 2122-2135 doi: 10.18632/aging.101536

2018 von Frieling J, Fink C, Hamm J, Klischies K, Forster M, Bosch TC, Roeder T, Rosenstiel P, Sommer F 
Grow with the challenge - microbial effects on epithelial proliferation, carcinogenesis and cancer therapy
Frontiers in Microbiology, doi: 10.3389/fmicb.2018.02020

2018 Stephano F, Nolte S, Hoffmann J, El-Kholy S, von Frieling J, Bruchhaus I, Fink C, Roeder T 
Impaired Wnt signaling in dopamine containing neurons is associated with pathogenesis in a rotenone triggered Drosophila Parkinson’s disease model.
Scientific Reports, 8(1):2372

2018   Romey-Glüsing R*, Li Y*, Hoffmann J, von Frieling J, Knop M, Pfefferkorn R, Bruchhaus I, Fink C, Roeder T​ 
Nutritional regimens with periodically recurring phases of dietary restriction extend lifespan in Drosophila
FASEB J​, accepted for publication

2017   Fink C, von Frieling J, Knop M, Roeder T 
Drosophila Fecal Sampling
Bio-protocols, accepted for publication

2017   Li Y, Tiedemann L, von Frieling J, Nolte S, El-Kholy S, Stephano F,  Gelhaus C, Bruchhaus I, Fink C, Roeder T (2017)
The role of monoaminergic neurotransmission for metabolic control in the fruit fly Drosophila melanogaster
Frontiers in Systems Neuroscience 1: 50. DOI=10.3389/fnsys.2017.00060

2016  Li Y, Hoffmann J, Li Y, Stephano F, Bruchhaus I, Fink C, Roeder T. Octopamine controls starvation resistance, life span and metabolic traits in Drosophila. Scientific Reports, accepted for publication

2016  Fink C, Hoffmann J, Knop M, Li Y, Isermann K, Roeder T. Intestinal FoxO signaling is required to survive oral infection in Drosophila. Mucosal Immunol. advance online publication

2016  Fink C, Roeder T. How well do surrogate hosts serve as model systems for understanding pathogenicity. Advances in Environmental   Microbiology, Ed. CJ Hurst.

2015  El-Kholy S, Stephano F, Li Y, Bhandari A, Fink C, Roeder T. Expression analysis of octopamine and tyramine receptors in Drosophila. Cell &   Tiss Res 361(3):669-684

2015  Oldefest M, Düsterhoft S, Desel C, Thysen S, Fink C, Rabe B, Lories R, Grötzinger J, Lorenzen I. Secreted Frizzled‐related Protein 3 (sFRP3)‐  mediated suppression of Interleukin‐6   receptor release by A disintegrin and metalloprotease 17 (ADAM17) is abrogated in the   osteoarthritisassociated rare double variant of sFRP3. Biochem J 468(3):507-518.

2015  Li Y, Fink C, El-Kholy S, Roeder T. The octopamine receptor octß2R is essential for ovulation and fertilization in the fruit fly Drosophila melanogaster. Arch Insect   Biochemistry & Physiology 88:168-178.

2014  Fink C, Schukies S, von Frieling J, Roeder T. Molekulare Analyse aminerger Signalwege im ZNS der Taufliege. Biospektrum 06.2014: 636-639.

2014  Faisal MN, Hoffmann J, El-Kholy S, Kallsen K, Wagner C, Bruchhaus I, Fink C, Roeder T. Transcriptional regionalization of the fruit fly’s airway epithelium. PLoS one 9:   e102534.

2013  Fink C, Staubach F, Kuenzel S, Baines JF, Roeder T. Non-invasive analysis of microbiome dynamics in the fruit fly Drosophila melanogaster.  Appl Environ Microbiol. 79:6984-6988.

2013  Hoffmann J, Romey R, Fink C, Yong, L, Roeder T . Overexpression of Sir2 in the adult fat body is sufficient to extend lifespan of male and f  emale   Drosophila. Aging 5: 315-327.

2013  Hoffmann J, Romey R, Fink C, Roeder T. Drosophila as a model to study metabolic disorders.  Adv Biochem Eng Biotechnol. 135: 41-61.
 
2006  von Wurmb-Schwark N, Mályusz V, Fremdt H, Koch C, Simeoni E, Schwark T. Fast and simple DNA extraction from saliva and sperm cells obtained from the skin or isolated from   swabs. Leg Med (Tokyo). May;8(3):177-81. Epub 2006 Mar 3
 

 

Dr. Jakob von Frieling

Postdoc

Phone: +49 431 880-4019
Telefax: +49 431 880-4197
jfrieling@zoologie.uni-kiel.de

Short CV

  • since 2015
PhD student, Kiel University, Germany
  • 2012 - 2015
Master studies, Kiel University, Germany
  Thesis: " Dopamine receptors in the Drosophila nervous system: physiological and behavioral aspects"
  • 2008 - 2012      
Bachelor studies, University of Göttingen, Germany
  Thesis: "Regulation der Corpora allata von Locusta migratoria durch die Neuropeptide Proctocolin und FMRFamid"

 

Research experience      

  
  • 2012/04 - 2012/09
Lab intern, Kiel University, Germany
  Project thesis: "Einfluss des Drosophila EGF-Rezeptors auf die Wundheilung und die Akkumulation von Triglyceriden"

 

Research topic

I am interested in effects of malnutrition and caloric restriction on epithelia-microbe interactions in the intestinal tract of Drosophila melanogaster. In detail, I will elucidate the contribution of epithelial cell-type specific alterations of candidate gene expression levels for maintaining intestinal immune homeostasis during malnutrition and caloric restriction. Furthermore, I try to answer the question if and how the identified signaling events in the epithelium and the changed microbiota shape inflammatory-like responses at the intestinal barrier.

Publications

von Frieling J, Faisal NM, Sporn F, Pefferkorn R, Nolte SS, Sommer F, Rosenstiel P, Roeder T (2020)
A high fat diet induces a microbiota dependent increase in stem cell activity in the Drosophila intestine.
PLOS Genetics, 16(5):e1008789, doi:10.1371/journal.pgen.1008789

Li Y, Romey-Glüsing R, Tahan Zadeh T, von Frieling J, Hoffmann J, Huebbe P, Bruchhaus I, Rimbach G, Fink C, Roeder T (2020)
Furbellow (brown algae) extract increases lifespan in Drosophila by interfering with TOR-signalling.
Nutrients, 12:1172, doi:10.3390/nu12041172

von Frieling J, Roeder T (2020)
Factors that affect the translation of dietary restriction into a longer life.
IUBMB Life, 72(5): 814-824, doi: http://doi.org/10.1002/iub.2224

Rausch P, Rühlemann M, Hermes B, Doms S, Dagan T, Dierking K, Domin H, Fraune S, von Frieling J, Hentschel-Humeida U, (...), Reusch TBH, Roeder T, Schmitz RA. Schulenburg H, Soluch R, Sommer F, Stuckenbrock E, Weiland-Bräuer N, Rosenstiel P, Franke A, Bosch T, Baines JF (2019)
Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms.
Microbiome, 7:133, doi: https://doi.org/10.1186/s40168-019-0743-1

von Frieling J, Fink C, Hamm J, Klischies K,Forster M, Bosch TC, Roeder T, Rosenstiel P, Sommer F (2018)
Grow with the challenge - microbial effects on epithelial proliferation, carcinogenesis and cancer therapy
Frontiers in Microbiology, doi: 10.3389/fmicb.2018.02020

Stephano F, Nolte S, Hoffmann J, El-Kholy S, von Frieling J, Bruchhaus I, Fink C, Roeder T (2018)
Impaired Wnt signaling in dopamine containing neurons is associated with pathogenesis in a rotenone triggered Drosophila Parkinson’s disease model.
Scientific Reports, 8(1):2372

Romey-Glüsing R*, Li Y*, Hoffmann J, von Frieling J, Knop M, Pfefferkorn R, Bruchhaus I, Fink C, Roeder T (2017)
Nutritional regimens with periodically recurring phases of dietary restriction extend lifespan in Drosophila
FASEB J​, 32: 1992-2002,. doi: 10.1096/fj.201700934R

Fink C, von Frieling J, Knop M, Roeder T (2017)
Drosophila Fecal Sampling
Bio-protocols, 7(18): e2547. DOI: 10.21769/BioProtoc.2547

Li Y, Tiedemann L, von Frieling J, Nolte S, El-Kholy S, Stephano F,  Gelhaus C, Bruchhaus I, Fink C, Roeder T (2017)
The role of monoaminergic neurotransmission for metabolic control in the fruit fly Drosophila melanogaster
Frontiers in Systems Neuroscience 1: 50. DOI=10.3389/fnsys.2017.00060

Fink C, Schukies S, von Frieling J, Roeder R (2014)
Molekulare Analyse aminerger Signalwege im ZNS der Taufliege.
BIOspektrum Volume 20, Issue 6: 636-639

Britta Laubenstein

Technician

Phone: +49 431 880-5151 / -3611
Telefax: +49 431 880-4197
blaubenstein@zoologie.uni-kiel.de

Dr. Mirjam Knop

Postdoc

R.807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
mknop@zoologie.uni-kiel.de

Alina Proske

PhD student

R.807
Phone: 0431 880 2581
aproske@zoologie.uni-kiel.de

Short CV

  • since 2020
PhD student, Kiel University, Germany
  • 2017 - 2020
Master studies, Kiel University, Germany
Thesis: "Impact of dietary and pharmacological interventions on
EGFR-induced Drosophila intestinal stem cell overproliferation"
  • 2014 - 2017
Bachelor studies, Kiel University, Germany
Thesis: "Analyse potentieller Einflussfaktoren auf die oxidative
Kapazität im menschlichen Speichel"

 

Research topic

Based on the results of my master thesis, I study the molecular effects of restricted protein intake on cell proliferation pathways involved in different Drosophila tumor models. Furthermore, I try to mimic the effects of the nutritional intervention by using pharmacological compounds.

 

Prof. Dr. Thomas Roeder

Professor

R.801
Phone: +49 431 880-4181
Telefax: +49 431 880-4197
troeder@zoologie.uni-kiel.de

CV

 

Christiane Sandberg

Techncian

Am Botanischen Garten 1-9, R.803 / 809
Phone: +49 431 880-5121 / -3611
Telefax: +49 431 880-4197
csandberg@zoologie.uni-kiel.de

Short CV

Working experience      

 
  • since 2009
MTLA, Department of Molecular Physiology, Kiel University
  • 2007 - 2008      
MTLA (Collaborative Research Center 617, Project A13), clinic for general internal medicine, University clinic Schleswig-Holstein
  • 1999 - 2001
MTLA (S2 laboratory), clinic for general surgery ward and thoracic surgery, Department of Molecular Oncology, University clinic Schleswig-Holstein
  • 1997 - 1999
MTLA (biochemical purification and functional characterisation of a protein), DFG project, Institute for Immunology, Kiel University
  • 1993 - 1997
Research assistance, Department of Biology, Kiel University
  • 1989 - 1993
MTLA, until 1990 in the routine laboratory, from 1991 on in the pancreas laboratory, I medical clinic, Kiel, Germany

 

Edcuation

 
  • 1993 - 1997
Studies in Biology, Kiel University, Germany
  major subject: microbiology, degree: diploma
  • 1987 - 1989
State-approved academy for technical assistans of medicine, Kiel University, Germany
  degree: state examination as MTLA

 

Navid Tahanzadeh

PhD student

R. 807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
Ntahanzadeh@zoologie.uni-kiel.de

Short CV

 

Research topic

Marine algae, which contain many biologically active compounds are my target of study. I endevaor to reveal algal extractions that have functional ingridients and to shed light on the mechanism of action of these algal extractions using Drosophila melanogaster as a read-out model. I am carrying out life span experiments with different concentrations of the corresponding algal extracts and a series of physiological experiments. I intent for evaluating which organ is the target of this intervention using tissue-specific RNAi experiments and looking into different genes, which show a clear age-associated increase increase in expression level.

Publications

Sharifi-Rad M, Tyeboon GS, Miri A, Sharifi-Rad M, Setzer WN, Fallah F, Kuhestani K, Tahanzadeh N, Sharifi-Rad J. Mutagenic, antimutagenic, antioxidant, anti-lipoxygenase and antimicrobial activities of Scandix pecten-veneris. Cell. Mol. Biol. 2016,62(6):9-16.doi:10.14715/cmb/2016.62.2

Niad M, Zaree S and Tahanzadeh N. Repsonse surface methodology for optimization of Cd(II) biosorption by Cytoseria myricaas. J. Biomed. 2016,1(2):e6264.doi:10.17795/jmb-6264

 

Lasse Tiedemann

PhD student

R.808
Phone: +49 431 880-4019
Telefax: +49 431 880-4197
tiedemann@zoologie.uni-kiel.de

Short CV

  • since 2016
PhD student, Kiel University, Germany
  • 2014 - 2016

Master studies, Kiel University, Germany
Thesis: "Evaluation of cell-type specific RNA isolation techniques from Drosophila melanogaster brain tissue"

  • 2010 - 2014      
Bachelor studies, Kiel University, Germany
  Thesis: "Localisation and regulation of PSI genes in Arabidopsis thaliana"

 

Research topic

Analysis of the transciptome of neuronal tissue, involved in hormonal regulation, particularly diuretic hormone 44 (Dh44)-expressing neurons in pars intercerebralis of the Drosophila brain. Furthermore, the establishment and comparison of cell type specific RNA isolation methods: TU-tagging and translating ribosome affinity purification (TRAP) in the Drosophila brain.

Publications


Finetti L, Tiedemann L, Zhang X, Civolani S, Bernacchia G, Roeder T (2020)
Monoterpenes alter TAR1-driven physiology in Drosophila species.
J exp Biol, accepted for publication

Li Y, Tiedemann L, von Frieling J, Nolte S, El-Kholy S, Stephano F,  Gelhaus C, Bruchhaus I, Fink C, Roeder T (2017)
The role of monoaminergic neurotransmission for metabolic control in the fruit fly Drosophila melanogaster
Frontiers in Systems Neuroscience 1: 50. DOI=10.3389/fnsys.2017.00060

Vaibhvi

PhD student

R.807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
vvaibhvi@zoologie.uni-kiel.de

Short CV

  • since 2019
PhD student, Kiel University, Germany
  • 2013-2018            

Integrated Bachelor's and Master's of Science, Indian Institute of Science Education and Research, Mohali, India

Master thesis: Role of cellular immune components in evolved immunity in Drosophila melanogaster

Research experience

2016/05-2016/07: Lab intern: Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India
                                 "Genetic analysis of a probable juvenile myoclonic epilepsy (JME) causing gene in indian population"

2015/05-2015/07: Lab intern: Indian Insitute of Science Education and Research, Bhubaneshwar, India
                                 "Molecular genetics of pseudoexfoliation syndrome and pseudoexfoliation glaucoma in indian population"

2014/05-2014/07: Lab intern: Indian Insitute of Science Education and Research, Mohali, India
                                 "Effect of dietary restriction on Drosophila melanogaster populations selected for differential rate of aging"

Research topic

I am highly fascinated by the effects of nutritional stress on the immunocompetence of animals. Using Drosophila melanogaster as a model, I work on deciphering the impact of different dietary compositions in terms of protein to carbohydrate ratios. Therefore, I examine various phenotypic and genotypic immune defense parameters, like survival ability on facing a pathogen, pathogen clearance and expression of genes involved in the immune system. Furthermore, I plan to extend my work to look for the existence of lifelong or trans-generational memory of nutritional stress and response to secondary stress encounters.

 

Xiaofu Wang

PhD student

R.811
Phone: +49 431 880-2621
Telefax: +49 431 880-4197

Short CV

 

Research topic

I am working on the generation of tumor models in the tracheal system of Drosophila for the identification of new targets for lung cancer treatment. Therefore, I manipulate different proto-oncogenes as well as tumor-supressor genes or combinations of both to generate tumor-like phenotypes of the tissue. By using these models, I am searching for unkown single compounds or combinations of compounds in different concentrations as a new treatment option.

 

Heidrun Wegner

Secretary

R.701
Phone: +49 431 880-4180
Telefax: +49 431 880-4197
hwegner@zoologie.uni-kiel.de

Xiaoying Zhang

PhD student

R.811
Phone: +49 431 880-2621
Telefax: +49 431 880-4197
xzhang@zoologie.uni-kiel.de

Short CV

Research experience

Research topic

I’m interested in using humanized Drosophila models to study metabolic diseases such as diabetes and obesity. Firstly, I produce humanized Drosophila suited for studying diabetes and obesity in the fly based on CRISPR/Cas9 technology and Gal4/UAS system. I will use the newly generated tools to experimentally interfere/induce obesity in the fly, then have a depth characterization of the influence of manipulation of hormonal signaling on diabetes and obesity.