Molecular Physiology

Team

Ali Al-Lami

PhD student

R.811
Phone: +49 431 880-2621
Telefax: +49 431 880-4197
aallami@zoologie.uni-kiel.de

Short CV

  • since 2014
PhD student, Kiel University, Germany
  • 1997
Master in animal physiology, University of Baghdad, Iraq
  • 1992
Bachelor in zoology, University of Baghdad, Iraq

 

Research topic

Drosophila oenocyte as a functional homolog of human hepatocyte. 

My topic highlight on the role of cyclic AMP as a second messenger in the oenocyte–lipid accumulation during starve condition, and its relation with metabolic diseases ,including diabetes and metabolic syndrome.

Judith Bossen

PhD student

R. 807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
jbossen@zoologie.uni-kiel.de

Short CV

  • since 2014
PhD student, Kiel University, Germany
  • 2011 - 2014      
Master studies, Kiel University, Germany
  Thesis: "Physiologische Rolle der Acyloxyacylhydrolase in Dictyostelium discoideum"
  • 2008 - 2011
Bachelor studies, Kiel University, Germany
  Thesis: "Analyse der Lysozym-Synthese bei Dictyostelium discoideum in der Abhängigkeit von der Gegenwart von Bakterien"

 

Research topic

I am working on the development and establishment of a lung cancer model in Drosophila. The aim is to understand the molecular and genetic basis of tumor formation and progression. Overexpression of cancer-associated genes (e.g. EGFR, Ras, MAPK) in the tracheal epithelium can induce cancer-like phenotypes like epithelial meta- and hyperplasia. I am investigating this phenotypes by microscopic analysis for differences on a cellular level (quantification of epithelial thickness, cell and nucleus size and number, cell connections and cell shape…) and by other methods (IHC, qPCR) to investigate the tumor formation on a translational and transcriptional level.

Publications

2015  Uliczka K, Bossen J, Pfefferkorn R, Heine H, Roeder T. Etablierung eines Drosophila-Lungenkarzinom-Modells. Pneumologie. 2015;69-A7

Christine Fink

Postdoc

R.808
Phone: +49 431 880-2578
Telefax: +49 431 880-4197
cfink@zoologie.uni-kiel.de

Short CV

  • since 2012
Postdoc, Kiel University, Germany
  • 2008 - 2012
PhD student, Kiel University, Germany
  Thesis: "Die Architektur des intestinalen Immunsystems der Taufliege Drosophila melanogaster"
  • 7/2009 - 7/2010      
Maternal leave
  • 2002 - 2008
Diploma studies in Biology, Kiel University, Germany
  Thesis: "Der Einfluss des Hungerns auf die Transkriptionsaktivität isolierter Organe der Taufliege Drosophila melanogaster"
 
 

Research topics

Based on results of my phD thesis I am investiganting the epithelial immune response in the fruit flys intestine. Especially the interaction between the microbiome and the host is not fully understood yet. Beside the classical antimicrobial peptide response via NF-kB pathways (Toll & Imd), I am interested in the DUOX response mechanisms that not only effects invading pathogens but even also the own microbiome. I want to understand how the host-microbiome-homeostatis is staying in balance.

Furthermore I am working with the airway epithelium of Drosophila. I am investiganting different epithelial phenotypes in the fly that model lifestyle diseases like  Asthma, COPD & lung cancer and try to investigate the underlying cell signalling mechanism that may cause the typical disease-like phenotypes. One major aspect is to test the influence of human suceptibility genes in the fly model to further investigate targets for a better treatment of this diseases.

Publications

2016  Li Y, Hoffmann J, Li Y, Stephano F, Bruchhaus I, Fink C, Roeder T. Octopamine controls starvation resistance, life span and metabolic traits in Drosophila. Scientific Reports, accepted for publication

2016  Fink C, Hoffmann J, Knop M, Li Y, Isermann K, Roeder T. Intestinal FoxO signaling is required to survive oral infection in Drosophila. Mucosal Immunol. advance online publication

2016  Fink C, Roeder T. How well do surrogate hosts serve as model systems for understanding pathogenicity. Advances in Environmental   Microbiology, Ed. CJ Hurst.

2015  El-Kholy S, Stephano F, Li Y, Bhandari A, Fink C, Roeder T. Expression analysis of octopamine and tyramine receptors in Drosophila. Cell &   Tiss Res 361(3):669-684

2015  Oldefest M, Düsterhoft S, Desel C, Thysen S, Fink C, Rabe B, Lories R, Grötzinger J, Lorenzen I. Secreted Frizzled‐related Protein 3 (sFRP3)‐  mediated suppression of Interleukin‐6   receptor release by A disintegrin and metalloprotease 17 (ADAM17) is abrogated in the   osteoarthritisassociated rare double variant of sFRP3. Biochem J 468(3):507-518.

2015  Li Y, Fink C, El-Kholy S, Roeder T. The octopamine receptor octß2R is essential for ovulation and fertilization in the fruit fly Drosophila melanogaster. Arch Insect   Biochemistry & Physiology 88:168-178.

2014  Fink C, Schukies S, von Frieling J, Roeder T. Molekulare Analyse aminerger Signalwege im ZNS der Taufliege. Biospektrum 06.2014: 636-639.

2014  Faisal MN, Hoffmann J, El-Kholy S, Kallsen K, Wagner C, Bruchhaus I, Fink C, Roeder T. Transcriptional regionalization of the fruit fly’s airway epithelium. PLoS one 9:   e102534.

2013  Fink C, Staubach F, Kuenzel S, Baines JF, Roeder T. Non-invasive analysis of microbiome dynamics in the fruit fly Drosophila melanogaster.  Appl Environ Microbiol. 79:6984-6988.

2013  Hoffmann J, Romey R, Fink C, Yong, L, Roeder T . Overexpression of Sir2 in the adult fat body is sufficient to extend lifespan of male and f  emale   Drosophila. Aging 5: 315-327.

2013  Hoffmann J, Romey R, Fink C, Roeder T. Drosophila as a model to study metabolic disorders.  Adv Biochem Eng Biotechnol. 135: 41-61.
 
2006  von Wurmb-Schwark N, Mályusz V, Fremdt H, Koch C, Simeoni E, Schwark T. Fast and simple DNA extraction from saliva and sperm cells obtained from the skin or isolated from   swabs. Leg Med (Tokyo). May;8(3):177-81. Epub 2006 Mar 3
 

 

Jakob von Frieling

PhD student

Phone: +49 431 880-4019
Telefax: +49 431 880-4197
jfrieling@zoologie.uni-kiel.de

Short CV

  • since 2015
PhD student, Kiel University, Germany
  • 2012 - 2015
Master studies, Kiel University, Germany
  Thesis: " Dopamine receptors in the Drosophila nervous system: physiological and behavioral aspects"
  • 2008 - 2012      
Bachelor studies, University of Göttingen, Germany
  Thesis: "Regulation der Corpora allata von Locusta migratoria durch die Neuropeptide Proctocolin und FMRFamid"

 

Research experience      

  
  • 2012/04 - 2012/09
Lab intern, Kiel University, Germany
  Project thesis: "Einfluss des Drosophila EGF-Rezeptors auf die Wundheilung und die Akkumulation von Triglyceriden"

 

Research topic

I am interested in effects of malnutrition and caloric restriction on epithelia-microbe interactions in the intestinal tract of Drosophila melanogaster. In detail, I will elucidate the contribution of epithelial cell-type specific alterations of candidate gene (NF-kB (Relish, Dorsal), Duox and Upd3) expression levels for maintaining intestinal immune homeostasis during malnutrition and caloric restriction. Furthermore, I try to answer the question if and how the identified signaling events in the epithelium and the changed microbiota shape inflammatory-like responses and carcinogenesis at the intestinal barrier. Additionally, I am interested  in the hormonal and aminergic regulation of the gut.

Publications

2014 Christine Fink, Stella Schukies, Jakob von Frieling, Thomas Roeder. Molekulare Analyse aminerger Signalwege im ZNS der Taufliege. BIOspektrum Volume 20, Issue 6: 636-639

Tian Gu

PhD student

R.811
Phone: +49 431 880-2621
Telefax: +49 431 880-4197
tgu@zoologie.uni-kiel.de

Short CV

  • since 2013
PhD student, Kiel University, Germany
  • 2009 - 2012      
Master studies, Northeast Agricultural University, China
  Thesis: "Directly fermentation of microcrystalline celluose to ethanol by using of Saccharomyces cerevisiae codisplaying of cellulytic enzyme"
  • 2005 - 2009
Bachelor studies, Northeast Agricultural University, China
  Thesis: "Comparison of three methods for total DNA extraction from soil microorganism"

 

Research topic

The unfolded protein response (UPR) is an intracellular signaling pathway ,associated with various neurodegenerative disorders, cancer and diabetes . My project is using Drosophila as a model , to study the role of three stress sensors in different UPR branches.

Interests: trachea,intestine,fat body/UPR signaling pathway ,ER-stress

Immunohistochemistry/Real-time PCR/ RNA isolation/Body fat quantification/Hypoxia treatment assay/Smoke treatment assay

Mohsen Hegab

PhD student

R.807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
mohsenhegab@daad-alumni.de

Short CV

  • since 2013
PhD student, Kiel University, Germany
  • 2009 - 2012      
Fulfilling the requirement relating to a permanent full-time research assistant position (DAAD-GERLS scholarship program), National Research Center of Egypt 
  • 2006 - 2009
M.Sc. degree in Biochemistry and Diploma of Molecular Biology and Genetic Engineering, Faculty of Science, Ain Shams University, Cairo, Egypt
  Thesis : "Molecular characterization of a Schistosoma Egg Antigen (SEA)-modulated genes in bladder cancer"
  • 2001 - 2005
Bachelor Science in Biology/Biochemistry, Faculty of Science, Ain Shams University
  Diploma of Clinical Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Egypt

 

Research topic

Adopting the evolutionary-conserved genes/mechanisms between vertebrates and the fruit fly Drosophila melanogaster to develop a translational model of human diseases with special interest in dysbiosis-associated inflammation in the gut.

Britta Laubenstein

Technician

Phone: +49 431 880-5151 / -3611
Telefax: +49 431 880-4197
blaubenstein@zoologie.uni-kiel.de

Mirjam Knop

PhD student

R.807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
mknop@zoologie.uni-kiel.de

Jan-Philip Kühle

Bachelor student

R.808
Phone: +49 431 880-4019
Telefax: +49 431 880-4197

Yuanyuan Ma

Phd student

R811
Phone: +49 431 880-2621
Telefax: +49 431 880-4197
yma@zoologie.uni-kiel.de

Short CV

  • since 2015
PhD student, Kiel University, Germany
  • 2012 - 2015
Master studies in Hydrobiology, Ocean University of China, China
  • 2008 - 2012
Bachelor studies, Shengyang Normal University, China

 

Reseach experience

 
  • 2012/09 - now
Studying complete genome of octopus
  • 2013/07 - 2013/09      
Exchange learning in Zhejiang province hospital of the integration of traditional chinese and western medicine
  • 2010/10 - 2011/10
College students' subject: toxicity effects of compound fertilizer on erythrocytes micronuclei and nuclear anomalies of Bufo raddes tadpoles studying at key laboratory of evolution and biodiversity
  • 2009/09 - 2010/09
National College Students Research Training Program

 

Research topic

I am interested in detecting aquatic xenobiotica of natural or anthropogenic origin via GFP tagged endogenously expressed Multi Drug Resistance transporters (MDR) of marine flatworms. I propose to develop a system using engineered aquatic flatworms for the detection of unknown substance classes that are present at very low concentrations.

Publications

Ma Y, Zheng X, Cheng R et al, 2014. The Complete Mitochondrial Genome of Octopus conispadiceus (Sasaki, 1917) (Cephalopoda: Octopodidae) . Mitochondria DNA. 27:1-2(IF=1.701, 2014)

Zheng X, Ma Y, Cheng R, 2014. Application of mitochondrial DNA in phylogenetic analysis of Cephalopods:A Review. Journal of Fisheries of China. doi:10.3724/SP.J.1231.2015.59518

Cheng R, Zheng X, Ma Y et al, 2013. The complete mitochondrial genomes of two Octopods Cistopus chinensis and Cistopus taiwanicus:revealing the phylogenetic position of Cistopus genus in octopoda order. PloS One, 8(12): e84216. (IF=3.534 , 2014)

Wang Y, Li P, Lu Y, Dai C, Wang Y, Yang H, Ma Y, 2011. Toxicity effects of compound fertilizer on erythrocytes micronuclei and nuclear anomalies of Bufo  raddei tadpoles.Chinese journal of ecology.

Ma Y, Zheng X, Cheng R, 2014. The complete mitochondrial genomes of two Octopus Cyanea. (In prep)

 

Stella Nolte

PhD student

R.807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
snolte@zoologie.uni-kiel.de

Short CV

  • since 2014
PhD student, Kiel University, Germany
  • 2013 - 2014
Research assistant, Kiel University, Germany
  • 2012
Maternity leave
  • 2012
Research assistant, MPI for Evolutionary Biology in Plön, Germany
  • 2011 - 2012
Diploma project, MPI for Evolutionary Biology in Plön, Germany
  Thesis: "Genetics of resistance to sigma virus in Drosophila"

 

Research topic

I am generally interested in the evolution of organ systems and regulatory mechanisms in metabolic pathways to match with changing environmental conditions. I am further interested in animal phylogeny and systematics.

For my PhD I am working on the dopaminergic system of the fruit fly. The focus of my research lies on the physiological role of dopamine receptors and the effects of Rotenone exposure on dopaminergic cells and their cellular processes.

To answer my research questions I am analysing the flies‘ behavioural and activity patterns, metabolic parameters and gene expression. I started working on my PhD in 2014 on the topic dopaminergic system of the fruit fly. 

Publications

2014  Fink C, Schukies S, von Frieling J, Roeder T. Molekulare Analyse aminerger Signalwege im ZNS der Taufliege. Biospektrum 06.2014: 636-639.

Roxana Pfefferkorn

PhD student

R.807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
rpfefferkorn@zoologie.uni-kiel.de

Short CV

  • since 2014
PhD student, Kiel University, Germany
  • 2011 - 2014      
Master studies, Kiel University, Germany
  Thesis: "Functional characterization of candidate genes identified from genetic analysis of experimentally evolved Pseudomonas aeruginosa"
  • 2008 - 2011
Bachelor studies, Kiel University, Germany
  Thesis: "Etablierung eines Zellkultursystems für Neuronen des dopaminergen Systems der Fruchtfliege Drosophila melanogaster"

 

Research experience      

 

 

  • 2010
Lab intern, Bernhard Nocht Institue for Tropical Medicince in Hamburg, Germany

 

Research topic

I am investigating the effect of nutrition on the development of intestinal cancer. The model I use induces uncontrolled stem cell proliferation due to an activation of the RAS/RAF/MAPK pathway. Additionally, I am researching the effect of cancer on the flys gut microbiota and vice versa.

The methods I use most frequently are: fluorescence microscopy, survival assays, luciferase assays, sequencing, statistics

Publications

2015   Jansen G, Crummenerl L, Gilbert F, Mohr T, Pfefferkorn R, Thänert R, Rosenstiel P, Schulenburg H. Evolutionary Transition from Pathogenicity to Commensalism: Global Regulator Mutations Mediate Fitness Gains through Virulence Attenuation. Mol Biol Evol. 2015 Nov;32(11):2883-96

2015  Uliczka K, Bossen J, Pfefferkorn R, Heine H, Roeder T. Etablierung eines Drosophila-Lungenkarzinom-Modells. Pneumologie. 2015;69-A/

2012  Chrobak M, Förster S, Meisel S, Pfefferkorn R, Förster F, Clos J. Leishmania donovani HslV does not   interact stably with HslU proteins. Int J Parasitol. 2012 Apr;42(4):329-39

Ruben Prange

Master student

R.808
Phone: +49 431 880-4019
Telefax: +49 431 880-4197
rprange@zoologie.uni-kiel.de

Short CV

  • since 2013
Master studies, Kiel University, Germany
  • 2010 - 2013
Bachelor studies, University of Hamburg, Germany
 
Thesis: "Establishment of a measuring method for a FRET-based biosensor on the Operetta high content imaging system"

 

Research experience      

 
  • 2015
Lab intern, Perkin Elmer in Hamburg, Germany
  Project thesis: "Comparative measurements concerning speed and quality of different high content imaging system"

 

Research topic

My research interest lies in the activation of COPD (Chronic Obstructive Pulmonary Disease) dependent signalling pathways in Drosophila. To apply Drosophila to cigarette smoke I am using a vacuum based method. After smoke exposure, the trachea of the flies must be dissected to further investigate potential COPD dependent activation motifs.

My mainly used methods to study the trachea of different fly strains are qRT-PCR and light/fluorescence microscopy. Moreover I am investigating the potential impact of myokines on the lifespan of smoke exposed flies. Myokines are secreted by a contracting muscle and are supposed to enhance the lifespan of other cells and therefore lead to longevity of a whole organism.

Due to the increasing mortality rate of this disease, the development of potential approaches to prevent or to milden the burden of COPD may become more and more important.

Publications

2016 Hans-Joachim Müller and Daniel Ruben Prange. PCR - Polymerase Kettenreaktion. Spinger-Verlag Berlin Heidelberg

2016 Foitzik A., Prange R., Böttcher K., Schreiner A. Opera Phenix High Conten Screening System Technical Performance: Speed. Perkin Elmer Technical Note

Reshmi Raveendran

PhD student

R.807
Phone: +49 431 880-2581
Telefax: +49 431 880-4197
rraveendran@zoologie.uni-kiel.de

Short CV

  • since 2013      
PhD student, Kiel University, Germany
  •  
Research assistant, Jawaharlal Nehru Center for Advanced Scientific
  Project thesis: "Circadian rhythm of egg laying behavior in Drosophila melanogaster"
  •  
Master studies in Biotechnology, Madurai Kamaraj University, India
  Thesis: " Molecular taxonomical identification of frogs in the western ghats using DNA barcode sequences"
  •  
Bachelor studies in Biotechnology, Madurai Kamaraj University, India

 

Research topics

For my PhD thesis, I work on the role of transcription factor FoxO in tissue homeostasis in Drosophila Melanogaster. My research mainly focus on plasticity of terminal tracheal cells and role of dFoxO in remodelling tracheal cells in response to hypoxia and nutrition.

Main experiments / skills : microscopy, immunohistochemistry, PCR based techniques,  Next Generation Sequence Analysis

Publications

2011 Kannan NN, Raveendran  R, Hari Dass S, Manjunatha T, Sharma VK. Temperature can entrain egg laying rhythm of Drosophila but may not be a stronger zeitgeber than light. Journal of Insect Physiology

 

Thomas Roeder

Professor

R.801
Phone: +49 431 880-4181
Telefax: +49 431 880-4197
troeder@zoologie.uni-kiel.de

CV

 

Christiane Sandberg

Techncian

Am Botanischen Garten 1-9, R.803 / 809
Phone: +49 431 880-5121 / -3611
Telefax: +49 431 880-4197
csandberg@zoologie.uni-kiel.de

Short CV

Working experience      

 
  • since 2009
MTLA, Department of Molecular Physiology, Kiel University
  • 2007 - 2008      
MTLA (Collaborative Research Center 617, Project A13), clinic for general internal medicine, University clinic Schleswig-Holstein
  • 1999 - 2001
MTLA (S2 laboratory), clinic for general surgery ward and thoracic surgery, Department of Molecular Oncology, University clinic Schleswig-Holstein
  • 1997 - 1999
MTLA (biochemical purification and functional characterisation of a protein), DFG project, Institute for Immunology, Kiel University
  • 1993 - 1997
Research assistance, Department of Biology, Kiel University
  • 1989 - 1993
MTLA, until 1990 in the routine laboratory, from 1991 on in the pancreas laboratory, I medical clinic, Kiel, Germany

 

Edcuation

 
  • 1993 - 1997
Studies in Biology, Kiel University, Germany
  major subject: microbiology, degree: diploma
  • 1987 - 1989
State-approved academy for technical assistans of medicine, Kiel University, Germany
  degree: state examination as MTLA

 

Lasse Tiedemann

PhD student

R.808
Phone: +49 431 880-4019
Telefax: +49 431 880-4197
tiedemann@zoologie.uni-kiel.de

Short CV

  • 2014 - 2016

Master studies, Kiel University, Germany
Thesis: "Evaluation of cell-type specific RNA isolation techniques from Drosophila melanogaster brain tissue"

  • 2010 - 2014      
Bachelor studies, Kiel University, Germany
  Thesis: "Localisation and regulation of PSI genes in Arabidopsis thaliana"

 

Research topic

Analysis of the transciptome of neuronal tissue, involved in hormonal regulation, particularly diuretic hormone 44 (Dh44)-expressing neurons in pars intercerebralis of the Drosophila brain. Furthermore, the establishment and comparison of cell type specific RNA isolation methods: TU-tagging and translating ribosome affinity purification (TRAP) in the Drosophila brain.

 

Marcus Thiedmann

Master student / PhD student

R.808
Phone: +49 431 880-4019
Telefax: +49 431 880-4197
mthiedmann@zoologie.uni-kiel.de

Short CV

  • since 2015      
Master studies, Kiel University, Germany
  • 2015
Bachelor studies in Applied Biology, University of Applied Sciences, Bonn-Rhein-Sieg, Germany
  Bachelor studies in Applied Sciences in Life Sciences, HAN University of Applied Sciences, Netherlands
  Thesis: "Quantification of cigarette smoke stress-induced in Drosophila tracheal epithelial cells by qPCR"

 

Research topic

Drosophila as a model for Chronic Obstructive Pulmonary Disease (COPD).

Heidrun Wegner

Secretary

R.701
Phone: +49 431 880-4180
Telefax: +49 431 880-4197
hwegner@zoologie.uni-kiel.de

Doaa Yamani

phD student

R. 811
Phone: +49 431 880-2621
Telefax: +49 431 880-4197
dyamani@zoologie.uni-kiel.de

Short CV

Resarch interest

I am interested in the molecular mechansims involved in COPD development using Drosophila melanogaster as model organism.

Publications

Wassim, N. M., Soliman, B. A., Yamani, D. F., I.M.A Nabi (2011). Rapid assay to differentiate the two forms of Egyptian Aedes caspius (Diptera: culicidae) using Acetylcolinesterase gene.J.Basic.Appl.Zool.66: 12–17.    

 

Xiao Niu

PhD student

R.811
Phone: +49 880-2621
Telefax: +49 431 880-4197
xniu@zoologie.uni-kiel.de

Short CV

  • since 2016
PhD student, Kiel University, Germany
  • 2005 - 2016
Working in the Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, China
  Thesis: " Dopamine receptors in the Drosophila nervous system: physiological and behavioral aspects"
  • 2012 - 2015     
Master studies in Hydrobiology, Ocean University of China, China
  • 2008 - 2012  
Bachelor studies, Shandong Polytechnic University, China

 

Research topic

My research interest lies in the mechanism of the development of asthma and COPD (Chronic Obstructive Pulmonary Disease). That utilizing of the newly generated tools, CRISPR/Cas9, to experimentally induce asthma or COPD-like phenotypes in the airway epithelia of the fly will make our research flies more similar to the human in the exxpression of disease genes, precluding the chances of original genes influencing the result.