Short CV

Research topic

I am working on the development and establishment of a lung cancer model in Drosophila. The aim is to understand the molecular and genetic basis of tumor formation and progression. Overexpression of cancer-associated genes (e.g. EGFR, Ras, MAPK) in the tracheal epithelium can induce cancer-like phenotypes like epithelial meta- and hyperplasia. I am investigating this phenotypes by microscopic analysis for differences on a cellular level (quantification of epithelial thickness, cell and nucleus size and number, cell connections and cell shape…) and by other methods (IHC, qPCR) to investigate the tumor formation on a translational and transcriptional level.

Publications

Bossen J, Uliczka K, Steen L, Neugebauer P, Mai M.M-Q, Fink C, Stracke F, Heine H and Roeder T (2020)
Driver mutations in major lung cancer oncogenes can be analyzed in Drosophila models.
ALTEX, doi: 10.14573/altex.1912131

Bossen J, Uliczka K, Steen L, Pfefferkorn R, Mai MM-Q, Burkhardt L, Spohn M, Bruchhaus I, Fink C, Heine H, Roeder T (2019)
An EGFR-induced Drosophila lung tumor model identifies alternative combination treatments.
Mol Cancer Ther, accepted for publication

Uliczka K, Bossen J, Pfefferkorn R, Heine H and Roeder T (2015)
Etablierung eines Drosophila-Lungenkarzinom-Modells.
Pneumologie, 69-A7

Short CV

Research topics

Based on results of my phD thesis I am investiganting the epithelial immune response in the fruit flys intestine. Especially the interaction between the microbiome and the host is not fully understood yet. Beside the classical antimicrobial peptide response via NF-kB pathways (Toll & Imd), I am interested in the DUOX response mechanisms that not only effects invading pathogens but even also the own microbiome. I want to understand how the host-microbiome-homeostatis is staying in balance.

Furthermore I am working with the airway epithelium of Drosophila. I am investiganting different epithelial phenotypes in the fly that model lifestyle diseases like  Asthma, COPD & lung cancer and try to investigate the underlying cell signalling mechanism that may cause the typical disease-like phenotypes. One major aspect is to test the influence of human suceptibility genes in the fly model to further investigate targets for a better treatment of this diseases.

Publications

2020   Bossen J, Uliczka K, Steen L, Neugebauer P, Mai M.M-Q, Fink C, Stracke F, Heine H and Roeder T
Driver mutations in major lung cancer oncogenes can be analyzed in Drosophila models.
ALTEX, doi: 10.14573/altex.1912131

2020   Niu X, Fink C, Kallsen K, Mincheva V, Franzenburg S, Prange R, Bossen J, Heine H, Roeder T 
Balanced JAK/STAT signaling is critical to maintain the functional and structural integrity of the Drosophila respiratory epithelium.
BioRxiv doi: https://doi.org/10.1101/2020.06.19.160929

2020   Richert E, Papenkort J, Klettner A, Tode J, Koinzer S, Brinkmann R, Fink C, Roeder T, Lucius R, Roider J
Response of Retinal Pigment Epithelium (RPE)-Choroid Explants to Thermal Stimulation Therapy of the RPE (TSR)
Lasers in Surgery and Medicine, accepted for publication DOI:10.1002/lsm.23288

2020   Li Y, Romey-Glüsing R, Tahan Zadeh T, von Frieling J, Hoffmann J, Huebbe P, Bruchhaus I, Rimbach G, Fink C, Roeder T 
Furbellow (brown algae) extract increases lifespan in Drosophila by interfering with TOR-signalling.
Nutrients, 12:1172, doi:10.3390/nu12041172

2019 Bossen J, Uliczka K, Steen L, Pfefferkorn R, Mai MM-Q, Burkhardt L, Spohn M, Bruchhaus I, Fink C, Heine H, Roeder T 
An EGFR-induced Drosophila lung tumor model identifies alternative combination treatments.
Mol Cancer Ther, accepted for publication

2018 Prange R*, Thiedmann M*, Bhandari A, Mishra N, Sinha A, Häsler R, Rosenstiel P, Uliczka K, Wagner C, Yldirim AÖ, Fink C, Roeder T 
A Drosophila model of cigarette smoke induced COPD identifies Nrf2 signaling as an expendient target for intervention
Aging, 10: 2122-2135 doi: 10.18632/aging.101536

2018 von Frieling J, Fink C, Hamm J, Klischies K, Forster M, Bosch TC, Roeder T, Rosenstiel P, Sommer F 
Grow with the challenge - microbial effects on epithelial proliferation, carcinogenesis and cancer therapy
Frontiers in Microbiology, doi: 10.3389/fmicb.2018.02020

2018 Stephano F, Nolte S, Hoffmann J, El-Kholy S, von Frieling J, Bruchhaus I, Fink C, Roeder T 
Impaired Wnt signaling in dopamine containing neurons is associated with pathogenesis in a rotenone triggered Drosophila Parkinson’s disease model.
Scientific Reports, 8(1):2372

2018   Romey-Glüsing R*, Li Y*, Hoffmann J, von Frieling J, Knop M, Pfefferkorn R, Bruchhaus I, Fink C, Roeder T​ 
Nutritional regimens with periodically recurring phases of dietary restriction extend lifespan in Drosophila
FASEB J​, accepted for publication

2017   Fink C, von Frieling J, Knop M, Roeder T 
Drosophila Fecal Sampling
Bio-protocols, accepted for publication

2017   Li Y, Tiedemann L, von Frieling J, Nolte S, El-Kholy S, Stephano F,  Gelhaus C, Bruchhaus I, Fink C, Roeder T (2017)
The role of monoaminergic neurotransmission for metabolic control in the fruit fly Drosophila melanogaster
Frontiers in Systems Neuroscience 1: 50. DOI=10.3389/fnsys.2017.00060

2016  Li Y, Hoffmann J, Li Y, Stephano F, Bruchhaus I, Fink C, Roeder T. Octopamine controls starvation resistance, life span and metabolic traits in Drosophila. Scientific Reports, accepted for publication

2016  Fink C, Hoffmann J, Knop M, Li Y, Isermann K, Roeder T. Intestinal FoxO signaling is required to survive oral infection in Drosophila. Mucosal Immunol. advance online publication

2016  Fink C, Roeder T. How well do surrogate hosts serve as model systems for understanding pathogenicity. Advances in Environmental   Microbiology, Ed. CJ Hurst.

2015  El-Kholy S, Stephano F, Li Y, Bhandari A, Fink C, Roeder T. Expression analysis of octopamine and tyramine receptors in Drosophila. Cell &   Tiss Res 361(3):669-684

2015  Oldefest M, Düsterhoft S, Desel C, Thysen S, Fink C, Rabe B, Lories R, Grötzinger J, Lorenzen I. Secreted Frizzled‐related Protein 3 (sFRP3)‐  mediated suppression of Interleukin‐6   receptor release by A disintegrin and metalloprotease 17 (ADAM17) is abrogated in the   osteoarthritisassociated rare double variant of sFRP3. Biochem J 468(3):507-518.

2015  Li Y, Fink C, El-Kholy S, Roeder T. The octopamine receptor octß2R is essential for ovulation and fertilization in the fruit fly Drosophila melanogaster. Arch Insect   Biochemistry & Physiology 88:168-178.

2014  Fink C, Schukies S, von Frieling J, Roeder T. Molekulare Analyse aminerger Signalwege im ZNS der Taufliege. Biospektrum 06.2014: 636-639.

2014  Faisal MN, Hoffmann J, El-Kholy S, Kallsen K, Wagner C, Bruchhaus I, Fink C, Roeder T. Transcriptional regionalization of the fruit fly’s airway epithelium. PLoS one 9:   e102534.

2013  Fink C, Staubach F, Kuenzel S, Baines JF, Roeder T. Non-invasive analysis of microbiome dynamics in the fruit fly Drosophila melanogaster.  Appl Environ Microbiol. 79:6984-6988.

2013  Hoffmann J, Romey R, Fink C, Yong, L, Roeder T . Overexpression of Sir2 in the adult fat body is sufficient to extend lifespan of male and f  emale   Drosophila. Aging 5: 315-327.

Short CV

Research topic

I am interested in effects of malnutrition and caloric restriction on epithelia-microbe interactions in the intestinal tract of Drosophila melanogaster. In detail, I will elucidate the contribution of epithelial cell-type specific alterations of candidate gene expression levels for maintaining intestinal immune homeostasis during malnutrition and caloric restriction. Furthermore, I try to answer the question if and how the identified signaling events in the epithelium and the changed microbiota shape inflammatory-like responses at the intestinal barrier.

Publications

von Frieling J, Faisal NM, Sporn F, Pefferkorn R, Nolte SS, Sommer F, Rosenstiel P, Roeder T (2020)
A high fat diet induces a microbiota dependent increase in stem cell activity in the Drosophila intestine.
PLOS Genetics, 16(5):e1008789, doi:10.1371/journal.pgen.1008789

Li Y, Romey-Glüsing R, Tahan Zadeh T, von Frieling J, Hoffmann J, Huebbe P, Bruchhaus I, Rimbach G, Fink C, Roeder T (2020)
Furbellow (brown algae) extract increases lifespan in Drosophila by interfering with TOR-signalling.
Nutrients, 12:1172, doi:10.3390/nu12041172

von Frieling J, Roeder T (2020)
Factors that affect the translation of dietary restriction into a longer life.
IUBMB Life, 72(5): 814-824, doi: http://doi.org/10.1002/iub.2224

Rausch P, Rühlemann M, Hermes B, Doms S, Dagan T, Dierking K, Domin H, Fraune S, von Frieling J, Hentschel-Humeida U, (...), Reusch TBH, Roeder T, Schmitz RA. Schulenburg H, Soluch R, Sommer F, Stuckenbrock E, Weiland-Bräuer N, Rosenstiel P, Franke A, Bosch T, Baines JF (2019)
Comparative analysis of amplicon and metagenomic sequencing methods reveals key features in the evolution of animal metaorganisms.
Microbiome, 7:133, doi: https://doi.org/10.1186/s40168-019-0743-1

von Frieling J, Fink C, Hamm J, Klischies K,Forster M, Bosch TC, Roeder T, Rosenstiel P, Sommer F (2018)
Grow with the challenge - microbial effects on epithelial proliferation, carcinogenesis and cancer therapy
Frontiers in Microbiology, doi: 10.3389/fmicb.2018.02020

Stephano F, Nolte S, Hoffmann J, El-Kholy S, von Frieling J, Bruchhaus I, Fink C, Roeder T (2018)
Impaired Wnt signaling in dopamine containing neurons is associated with pathogenesis in a rotenone triggered Drosophila Parkinson’s disease model.
Scientific Reports, 8(1):2372

Romey-Glüsing R*, Li Y*, Hoffmann J, von Frieling J, Knop M, Pfefferkorn R, Bruchhaus I, Fink C, Roeder T​ (2017)
Nutritional regimens with periodically recurring phases of dietary restriction extend lifespan in Drosophila
FASEB J​, 32: 1992-2002,. doi: 10.1096/fj.201700934R

Fink C, von Frieling J, Knop M, Roeder T (2017)
Drosophila Fecal Sampling
Bio-protocols, 7(18): e2547. DOI: 10.21769/BioProtoc.2547

Li Y, Tiedemann L, von Frieling J, Nolte S, El-Kholy S, Stephano F,  Gelhaus C, Bruchhaus I, Fink C, Roeder T (2017)
The role of monoaminergic neurotransmission for metabolic control in the fruit fly Drosophila melanogaster
Frontiers in Systems Neuroscience 1: 50. DOI=10.3389/fnsys.2017.00060

Fink C, Schukies S, von Frieling J, Roeder R (2014)
Molekulare Analyse aminerger Signalwege im ZNS der Taufliege.
BIOspektrum Volume 20, Issue 6: 636-639

Short CV

Research topic

Based on the results of my master thesis, I study the molecular effects of restricted protein intake on cell proliferation pathways involved in different Drosophila tumor models. Furthermore, I try to mimic the effects of the nutritional intervention by using pharmacological compounds.

CV

Short CV

Short CV

Research topic

Marine algae, which contain many biologically active compounds are my target of study. I endevaor to reveal algal extractions that have functional ingridients and to shed light on the mechanism of action of these algal extractions using Drosophila melanogaster as a read-out model. I am carrying out life span experiments with different concentrations of the corresponding algal extracts and a series of physiological experiments. I intent for evaluating which organ is the target of this intervention using tissue-specific RNAi experiments and looking into different genes, which show a clear age-associated increase increase in expression level.

Publications

Sharifi-Rad M, Tyeboon GS, Miri A, Sharifi-Rad M, Setzer WN, Fallah F, Kuhestani K, Tahanzadeh N, Sharifi-Rad J. Mutagenic, antimutagenic, antioxidant, anti-lipoxygenase and antimicrobial activities of Scandix pecten-veneris. Cell. Mol. Biol. 2016,62(6):9-16.doi:10.14715/cmb/2016.62.2

Niad M, Zaree S and Tahanzadeh N. Repsonse surface methodology for optimization of Cd(II) biosorption by Cytoseria myricaas. J. Biomed. 2016,1(2):e6264.doi:10.17795/jmb-6264

Short CV

Research topic

Analysis of the transciptome of neuronal tissue, involved in hormonal regulation, particularly diuretic hormone 44 (Dh44)-expressing neurons in pars intercerebralis of the Drosophila brain. Furthermore, the establishment and comparison of cell type specific RNA isolation methods: TU-tagging and translating ribosome affinity purification (TRAP) in the Drosophila brain.

Publications

Finetti L, Tiedemann L, Zhang X, Civolani S, Bernacchia G, Roeder T (2020)
Monoterpenes alter TAR1-driven physiology in Drosophila species.
J exp Biol, accepted for publication

Li Y, Tiedemann L, von Frieling J, Nolte S, El-Kholy S, Stephano F,  Gelhaus C, Bruchhaus I, Fink C, Roeder T (2017)
The role of monoaminergic neurotransmission for metabolic control in the fruit fly Drosophila melanogaster
Frontiers in Systems Neuroscience 1: 50. DOI=10.3389/fnsys.2017.00060

Short CV

Research experience

2016/05-2016/07: Lab intern: Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India
                                 "Genetic analysis of a probable juvenile myoclonic epilepsy (JME) causing gene in indian population"

2015/05-2015/07: Lab intern: Indian Insitute of Science Education and Research, Bhubaneshwar, India
                                 "Molecular genetics of pseudoexfoliation syndrome and pseudoexfoliation glaucoma in indian population"

2014/05-2014/07: Lab intern: Indian Insitute of Science Education and Research, Mohali, India
                                 "Effect of dietary restriction on Drosophila melanogaster populations selected for differential rate of aging"

Research topic

I am highly fascinated by the effects of nutritional stress on the immunocompetence of animals. Using Drosophila melanogaster as a model, I work on deciphering the impact of different dietary compositions in terms of protein to carbohydrate ratios. Therefore, I examine various phenotypic and genotypic immune defense parameters, like survival ability on facing a pathogen, pathogen clearance and expression of genes involved in the immune system. Furthermore, I plan to extend my work to look for the existence of lifelong or trans-generational memory of nutritional stress and response to secondary stress encounters.

Short CV

Research topic

I am working on the generation of tumor models in the tracheal system of Drosophila for the identification of new targets for lung cancer treatment. Therefore, I manipulate different proto-oncogenes as well as tumor-supressor genes or combinations of both to generate tumor-like phenotypes of the tissue. By using these models, I am searching for unkown single compounds or combinations of compounds in different concentrations as a new treatment option.

Short CV

Research experience

Research topic

I’m interested in using humanized Drosophila models to study metabolic diseases such as diabetes and obesity. Firstly, I produce humanized Drosophila suited for studying diabetes and obesity in the fly based on CRISPR/Cas9 technology and Gal4/UAS system. I will use the newly generated tools to experimentally interfere/induce obesity in the fly, then have a depth characterization of the influence of manipulation of hormonal signaling on diabetes and obesity.